Composite
Part:BBa_K4501020:Experience
Designed by: Pau Marín Escudero Group: iGEM22_Barcelona_UB (2022-09-22)
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Applications of BBa_K4501020
We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA.
Figure 1. (A) Results of qPCR for Ramos and HEK293T cell line. (B) GFP expression of the cell lines transfected with the constructions.
User Reviews
UNIQ9abc7a5c397b7754-partinfo-00000000-QINU UNIQ9abc7a5c397b7754-partinfo-00000001-QINU